Soluble DHFR expression degrees in molecules for every mobile calculated from lysate activity assays as described in resources and strategies.
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Protein mutational landscapes are shaped by the cellular setting, but critical components as well as their quantitative results are sometimes unfamiliar. listed here we exhibit that Lon, an excellent Command protease By natural means absent in prevalent E. coli expression strains, considerably reshapes the mutational landscape of your metabolic enzyme dihydrofolate reductase (DHFR). variety less than problems that solve really active mutants reveals that 23.
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